Teiko

The TokuKit is Teiko’s whole blood collection and preservation kit, designed for mass and spectral flow cytometry in research studies and clinical trials. It simplifies blood sample preservation with a quick, 3-step process—collect, fix, and freeze—that takes 20 minutes or less. No specialized training or equipment is needed.

Samples processed with the TokuKit are frozen at -80°C and remain stable for months. Blood samples preserved with the TokuKit are compatible with conventional flow, spectral flow, and mass cytometry. Unlike PBMC isolation, the TokuKit preserves granulocytes—neutrophils, basophils, and mast cells—enabling a more complete profile of the immune system.

Learn more at teiko.bio/tokukit.

We recommend collecting whole blood into standard vacutainers containing—in order of preference—heparin or EDTA, and using our three-step TokuKit for on-site processing in clinical trials. This approach is faster, easier, and more cost-effective than PBMC isolation, with no centrifugation steps, and yields high-quality cytometry data with sample stability for months at -80°C.

We cannot accept whole blood collected in RNA or DNA isolation tubes, or serum samples, as these lack intact cells required for cytometry-based analysis.

We conducted an inter-kit precision study and observed an average coefficient of variation (CV) of 5.76% across all 33 immune cell populations analyzed. All populations were within the industry-standard 25% CV cutoff.

To assess inter-kit precision, we used samples from three donors, split one sample from each donor into three replicates, and fixed each of the nine samples using a separate TokuKit. We then analyzed the samples using our CLIA-validated, off-the-shelf, 40+ marker whole blood mass cytometry panel.

We recommend processing samples within 5 hours of the blood draw.

The collected blood can remain at room temperature in the vacutainer for up to 5 hours before processing with the TokuKit. We found that 100% of immune cell populations are preserved when TokuKits are processed within 5 hours, but that delays to 7 hours or more results in loss of several important immune lineages.

Generally speaking, the sooner the blood is processed with the TokuKit after blood draw the better. If possible, we recommend processing within 2 hours of blood collection when possible.

Learn more at teiko.bio/tokukit.

Blood samples processed with Teiko's TokuKits can be sent directly to our CLIA-compliant laboratory in Salt Lake City, Utah. They can be received Monday–Friday, 9 a.m.–5 p.m.

Address:
675 Arapeen Drive, Suite 301
Salt Lake City, UT 84108
ATTN: Li-Chun Cheng or Teiko Lab